TSRHC

Research

Cellular Pathology

The division of Cellular Pathology at Texas Scottish Rite Hospital for Children processes tissue specimens for clinical diagnostics and research applications. The division consists of a highly experienced group of physicians, histotechnologists, laboratory technicians, scientists and other specialists.

The Cellular Pathology team applies the studies of basic science and clinical research to a different range of services, including:

Routine Histology
Histology refers to the microscopic structure of tissues. The department processes tissue specimens, including bone, skin, nerve and muscle biopsies, for routine histological preparations, histochemistry and enzyme staining. Specimens are processed for clinical studies and research applications to explore fundamental questions related to biology of normal cellular growth and differentiation.

Rapid Frozen Section
The Cellular Pathology staff is highly experienced in the Rapid Frozen Technique used to perform an immediate microscopic interpretation of tissue. The procedure is performed in the operating room for an accurate diagnosis and decision about intraoperative and postoperative management.

Immunocytochemistry
The department performs immunocytochemical staining to identify cellular or tissue constituents by means of antigen-antibody binding interactions. Different chromogens (secondary antibodies) bind to the primary antibody for imaging, creating a colorful tissue preparation when examined on a light microscope or fluorescent microscope. The department also performs in-situ hybridization, a procedure that uses a complementary strand of DNA or RNA to localize a specific DNA or RNA sequence in a section of tissue.

Histomorphometry
The department provides histomorphometric analysis, the quantitative study of a tissue’s structure and microscopic organization. The analysis provides a quantitative expression and measurement of the patterns present in sections of bone, muscle and nerve to assess the severity of diseases and research tissue formation.

Electron Microscopy
Electron microscopy uses electrons to illuminate an image and create an enlarged view with a greater resolution than that of a light microscope. The department processes and stains tissue for Transmission Electron Microscopy (TEM) specimens. This allows researchers to interpret and understand the structure and function of cells, as well as tissue ultrastructure, beyond what is seen with a conventional light microscope.

Hard Tissue Techniques
A specialized area of the department processes mineralized bone embedded in Methyl Methacrylate (MMA) that matches the hardness of the bone and permits undisrupted sections. The procedure allows researchers to examine bone density or defects.

Neuropathology
Neuropathology is the study of disease in the nervous system tissue, usually performed through surgical biopsies. Skeletal muscle biopsies and peripheral nerve biopsies are processed in the Cellular Pathology department according to strict protocols that require special handling.

For more information about TSRHC’s Cellular Pathology department please call (214) 559-7766 or (800) 421-1121,
ext. 7766, or e-mail Reuel.Cornelia@tsrh.org.